PCR

Invitrogen HiFi Platinum Taq

Component	Volume
10X Buffer 10 mM dNTPs 50 mM MgSO₄ Primer (10µM) Primer (10µM) DNA Taq ddH₂O	5 1 2 1 1 x 0.2 to 50

Recommended cycling parameters:

Denature 94°C for 15 - 30 s
Anneal 55°C for 15 - 30 s
Extend 68°C for 1 min per kb.

Component	Volume
10X Buffer 2 mM dNTPs 25 mM MgSO₄ 5' primer (10µM) 3' primer (10µM) DNA KOD (1 U/µl) ddH₂O	5 5 2-4 1.5 1.5 x 1 to 50

Recommended cycling parameters:

Activate the enzyme by incubating at 94°C for 2 min.
Perform 30-40 cycles:
- Denature 15 sec at 94°C
- Anneal 30 sec at primer Tm
- Extend 68°C for 1 min per kb.
Finish with 5 min at 68°C